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KMID : 0620920000320020067
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Experimental & Molecular Medicine
2000 Volume.32 No. 2 p.67 ~ p.71
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Major house dust mite allergen, Der p I, activates phospholipase D in human peripheral blood mononuclear cells from allergic patients: Involvement of protein kinase C
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Joong Soo Han/Jae Won Oh
Jong Hoon Kim/Ki Sung Lee/Joong Soo Han
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Abstract
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The major house-dust-mite allergen, Der p I, stimulates the phospholipase D (PLD) in peripheral blood mononuclear cells (PBMC) from allergic patients with maximal responses after 30 min exposure. At 30 min, Der p I stimulated PLD activity by 1.4-fold in mild, 1.6-fold in moderate and 2-fold in severe allergic patients over control values (p < 0.05). When the cells were pretreated for 24 h with phorbol myristate acetate to down-regulate protein kinase C (PKC), PLD stimulation by Der p I was largely abolished. These results indicate that in PBMC from allergic patients, Der p I can stimulate PLD activity, and that PKC activation is involved in this stimulation.
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KEYWORD
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Der p I, phospholipase D, protein kinase C, allergy, PBMC,
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